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Bioss
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Bioss
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The CD8 antigen, also known as T8 or Leu2 or Lyt2 or T cell coreceptor, is a cell surface glycoprotein as a dimer with a molecular mass of 32 KD. It is found on most
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Journal:
Article Title: KDEL and KKXX Retrieval Signals Appended to the Same Reporter Protein Determine Different Trafficking between Endoplasmic Reticulum, Intermediate Compartment, and Golgi Complex
doi: 10.1091/mbc.E02-08-0468
Figure Lengend Snippet: Different presence of CD8-K and CD8-E19 at ER exit sites in transiently transfected HuH-7 cells. Conventional thin section electron microscopy (a and b) and cryoimmunoelectron microscopy (c–k) of parental (a and b) and transiently transfected HuH-7 cells expressing CD8-K (c–f) and CD8-E19 (g–k) proteins. The ultrastructural analysis revealed the presence of long ER cisternae and outer nuclear membranes mostly studded with ribosomes (arrowheads in a and b) and occasionally showing smooth areas with emerging protrusions (arrows in a and b) facing IC units (asterisk in a). Immunogold labeling with anti-CD8 polyclonal antibody and protein A-colloidal gold conjugates were dense and unevenly distributed over ER cisternae (c), whereas the gold particles frequently were localized on the smooth ribosome-free areas of the membranes and clustered over protrusions extending from them (e and f, arrows). Similar clustering of gold particles was observed in protrusions extending from the outer nuclear membranes (d, arrow). Immunolabeling was also present on IC units (d, asterisk). In contrast, immunogold labeling of CD8-E19–expressing cells was homogeneously distributed over either outer nuclear membranes (g) or ER cisternae (k) and the protrusions extending from the ER (arrows in h–j) were often unlabeled but sometimes labeled (j). M, mitochondria; er, endoplasmic reticulum; NM, nuclear membrane; Nu, nucleus. Bars, 0.1 μm.
Article Snippet: Ultrathin cryosections were collected with sucrose and methylcellulose and incubated with
Techniques: Transfection, Electron Microscopy, Microscopy, Expressing, Labeling, Immunolabeling